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KMID : 0615720020190020028
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Jouranl of Korean Acupuncture and Moxibustion society
2002 Volume.19 No. 2 p.28 ~ p.38
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The Effect of Bee Venom & Purified Bee Venom on Cell Death in Synovial Cell
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Abstract
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Objective : This study is aimed to investigate the effects of bee venom and purified bee venom on cell death in synovial cell line.
Methods : It was evaluated by using MTT assay, morphological method, flow cytometry, immunocytochemistry analysis, RT-PCR.
Results : The result obtained is as follows.
1. The MIT assay demonstrated that synovial cell viability was significantly inhibitted dose-dependently by treatment with BV and PBV in comparison with control. And the inhibitory effect of BV and PBV was almost same.
2. The morphologic study demonstrated that synovial cell showed apoptotic body resulted from apoptosis after treatment with BV and PBV for 6 hours using microscope.
3. The Flow cytometry demonstrated that apoptosis of synovial cell treated with BV and PBV was related with stop of cell cycle in stage of G0/G1.
4. Immunocytochemistry assay demonstrated that COX-Ⅱand iNOS were slightly expressed by treatment with BV and PBV in comparison with control group.
5. RT-PCR analysis demonstrated that COX-Ⅱ were almost down-regulated by high dose treatment with BV and PBV in comparison with control group. iNOS were well down-regulated by treatment with 5㎍/ml BV and PBV whereas it was well expressed in control group.
Conclusion : These results suggest that bee venom and purified bee venom have significant effect on cell death in synovial cell line and further study is needed in vivo.
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KEYWORD
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